Journal:

Article Title: Acquisition of Plasmin Activity by Fusobacterium nucleatum subsp. nucleatum and Potential Contribution to Tissue Destruction during Periodontitis

doi:

Figure Lengend Snippet: Degradation of TIMP-1 by u-PA-activated plasminogen-coated F. nucleatum subsp. nucleatum. Bacteria were incubated with TIMP-1 for 16 h at 37°C, and the supernatants were analyzed by SDS-PAGE and Western immunoblotting using an anti-TIMP-1 antibody. Lanes: 1, molecular mass markers (kDa); 2, TIMP-1 alone; 3, u-PA-activated plasminogen-coated bacteria with TIMP-1; 4, pure plasmin with TIMP-1; and 5, plasminogen-coated bacteria with TIMP-1.

Article Snippet: Thereafter, the membrane was incubated for 2.5 h with rabbit anti-native human TIMP-1 (Cedarlane Laboratories Ltd.) as the primary antibody at a concentration of 4 μg/ml, and then with goat anti-rabbit immunoglobulin G alkaline phosphatase conjugate (Bio-Rad Laboratories, Mississauga, Ontario, Canada) as the secondary antibody at a dilution of 1/3,000 for 60 min.

Techniques: Incubation, SDS Page, Western Blot